The California congresswoman has been a vocal supporter of Joe Biden, the US president, over his handling of the conflict.
Mr Biden has stated his “iron clad” support for the defence of Israel since the October 7 attacks, but called for Mr Netanyahu to show restraint in his pursuit of Hamas and to allow humanitarian aid into Gaza.
“The biggest advocate for humanitarian assistance to the Palestinians has been Joe Biden, he’s the only one advocating at that level,” Ms Pelosi said.
She also expressed concerns about a push by some European countries, including the UK, to recognise a Palestinian state when the war has ended.
“I don’t know what state they would be recognising – a Hamas-ruling state or the Abbas Palestinian authority,” she said.